HTB-119 NCI-H69 H69人肺小細胞肺癌細胞

簡要描述：HTB-119 NCI-H69 [H69]人肺小細胞肺癌細胞
HTB-119 NCI-H69 H69人肺小細胞肺癌細胞
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詳細介紹

HTB-119 NCI-H69 [H69]人肺小細胞肺癌細胞

ATCC^® N

AF Gazdar

Bios

umber:

HTB-119™

Price:

Designations:

NCI-H69 [H69]

Depositors:

1
Shipped:	frozen
Medium & Serum:	See Propagation

afety Level:

Growth Properties:

suspension, multicell aggregates

Organism:

Homo sapiens

Morphology:

floating aggregates

Source:

Organ: lung
Disease: carcinoma; small cell lung cancer

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors:

insulin-like growth factor II （IGF II）

Tumorigenic:

Yes HTB-119 NCI-H69 [H69]人肺小細胞肺癌細胞

Oncogene:

myc +; myb +; fes +; fms +; raf +; ras +

DNA Profile （STR）:

CSF1PO: 10, 12

D13S317: 12

D16S539: 11

D5S818: 11, 13

D7S820: 9

THO1: 8, 9

TPOX: 10

vWA: 16, 17

Amelogenin: XY

Cytogenetic Analysis:

modal number = 76 to 78; range = 40 to 87

This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der（16）t（1;16）（q21;q23）, der（22）t（4;22）（q12;q13）, der（12）t（11;12）（q23;p12）, del（17）（p11）, der（19）t（5;19）（?q21;q13） and others.

Isoenzymes:

AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 2

PGM3, 1

Age:

55 years adult HTB-119 NCI-H69 [H69]人肺小細胞肺癌細胞

Gender:

male

Ethnicity:

Caucasian

Comments:

This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.

The cells grow in aggregates, thus cell counts are not accurate.

The cells stain positively for cytokeratins.

The line can be adapted to grow in shaker flask or spinner flask systems.

The N-myc gene is amplified, and there is expression of the mRNA and protein.

C-myc mRNA, but not protein, is expressed at a low level.

There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.

Propagation:

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C

Subculturing:

Protocol: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended HTB-119 NCI-H69 [H69]人肺小細胞肺癌細胞
Medium Renewal: 2 times weekly

Preservation:

Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature

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