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TIB-192
Comments:
Established in vitro from spontaneous myeloid leukemia of SL strain mice.
Can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone, LPS and WEHI-3 conditioned medium.
Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References:
1220: Ralph P, et al. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J. Immunol. 130: 108-114, 1983. PubMed: 6847876
22710: Ichikawa Y. Differentiation of a cell line of myeloid leukemia. J. Cell. Physiol. 74: 223-234, 1969. PubMed: 4187945
33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 （spi-1） promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022
