immunoglobulin; monoclonal antibody; against human acetylcholinesterase
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AE-2 小鼠雜交瘤細胞抗AChE
Comments:
Animals were immunized with purified human erythrocyte acetylcholinesterase.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with human and monkey neuromuscular junctions.
Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:
939: Fambrough DM, et al. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies
