Organ: bone Strain: C57BL/6 Tissue: calvaria Cell Type: preosteoblast;
Cellular Products:
collagen [51540]
Permits/Forms:
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Tumorigenic:
Yes
Age:
newborn newborn
Comments:
A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 （ATCC CRL-2593） and the MC3T3 Subclone 14 （ATCC CRL-2594） lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix （ECM） after 10 days [PubMed ID: 10352097].
The MC3T3 Subclone 24 （ATCC CRL-2595） and the MC3T3 Subclone 30 （ATCC CRL-2596） lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14 [PubMed ID: 10352097].
Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein （BSP）, osteocalcin （OCN）, and the parathyroid hormone （PTH）/parathyroid hormone-related protein （PTHrP） receptor.S ubclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor [PubMed ID: 10352097].
